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Uric acid is oxidised by the action of uricase to allantoin and hydrogen peroxide. In the presence of peroxidase (POD) the mixture of dichlorophenol sulphonate (DCBS) and 4-aminoantipyrine (4-AA) are condensed by the action of hydrogen peroxide, forming a coloured quinonaimine proportional to the concentration of uric acid in the sample.
Uric acid is oxidised by the action of uricase to allantoin and hydrogen peroxide. In the presence of peroxidase (POD) the mixture of dichlorophenol sulphonate (DCBS) and 4-aminoantipyrine (4-AA) are condensed by the action of hydrogen peroxide, forming a coloured quinonaimine proportional to the concentration of uric acid in the sample.
Uric acid is oxidised by the action of uricase to allantoin and hydrogen peroxide. In the presence of peroxidase (POD) the mixture of dichlorophenol sulphonate (DCBS) and 4-aminoantipyrine (4-AA) are condensed by the action of hydrogen peroxide, forming a coloured quinonaimine proportional to the concentration of uric acid in the sample.