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Urea is hydrolysed by urease to ammonia and carbon dioxide. Ammonia is converted to glutamate by glutamate dehydrogenase (GlDH) in the presence of NADH and ketoglutarate. The reaction is measured kinetically at 340 nm through the decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to the concentration of urea present in the sample.
Urea is hydrolysed by urease to ammonia and carbon dioxide. Ammonia is converted to glutamate by glutamate dehydrogenase (GlDH) in the presence of NADH and ketoglutarate. The reaction is measured kinetically at 340 nm through the decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to the concentration of urea present in the sample.
Urea is hydrolysed by urease to ammonia and carbon dioxide. Ammonia is converted to glutamate by glutamate dehydrogenase (GlDH) in the presence of NADH and ketoglutarate. The reaction is measured kinetically at 340 nm through the decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to the concentration of urea present in the sample.
Uric acid is oxidised by the action of uricase to allantoin and hydrogen peroxide. In the presence of peroxidase (POD) the mixture of dichlorophenol sulphonate (DCBS) and 4-aminoantipyrine (4-AA) are condensed by the action of hydrogen peroxide, forming a coloured quinonaimine proportional to the concentration of uric acid in the sample.
Uric acid is oxidised by the action of uricase to allantoin and hydrogen peroxide. In the presence of peroxidase (POD) the mixture of dichlorophenol sulphonate (DCBS) and 4-aminoantipyrine (4-AA) are condensed by the action of hydrogen peroxide, forming a coloured quinonaimine proportional to the concentration of uric acid in the sample.
Uric acid is oxidised by the action of uricase to allantoin and hydrogen peroxide. In the presence of peroxidase (POD) the mixture of dichlorophenol sulphonate (DCBS) and 4-aminoantipyrine (4-AA) are condensed by the action of hydrogen peroxide, forming a coloured quinonaimine proportional to the concentration of uric acid in the sample.