Cromatest

The method is based on the specific binding of bromocresol green (VBC), an anionic dye, and the protein at an acidic pH, resulting in a shift in the absorption spectrum of the complex. The intensity of the formed color is proportional to the concentration of albumin in the sample.

The method is based on the specific binding of bromocresol green (VBC), an anionic dye, and the protein at an acidic pH, resulting in a shift in the absorption spectrum of the complex. The intensity of the formed color is proportional to the concentration of albumin in the sample.

Alkaline phosphatases (FAL) catalyze the hydrolysis of 4-nitrophenyl phosphate (4-NFF) to form 4-nitrophenol and inorganic phosphate, with the alkaline buffer acting as a phosphate acceptor. The reaction is kinetically monitored at 405 nm based on the rate of 4-nitrophenol formation, proportional to the FAL activity present in the sample.

Alkaline phosphatases (FAL) catalyze the hydrolysis of 4-nitrophenyl phosphate (4-NFF) to form 4-nitrophenol and inorganic phosphate, with the alkaline buffer acting as a phosphate acceptor. The reaction is kinetically monitored at 405 nm based on the rate of 4-nitrophenol formation, proportional to the FAL activity present in the sample.