Cromatest

Lactate dehydrogenase (LDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH). The reaction is kinetically monitored at 340 nm through the decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to ALT activity in the sample.

Lactate dehydrogenase (LDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH). The reaction is kinetically monitored at 340 nm through the decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to ALT activity in the sample.

Lactate dehydrogenase (LDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH). The reaction is kinetically monitored at 340 nm through the decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to ALT activity in the sample.

Lactate dehydrogenase (LDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH). The reaction is kinetically monitored at 340 nm through the decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to ALT activity in the sample.

Aspartate aminotransferase (AST/GOT) catalyzes the transfer of the amino group from aspartate to alpha-ketoglutarate, forming glutamate and oxaloacetate. The latter is reduced to malate by malate dehydrogenase (MDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH). The reaction is kinetically monitored at 340 nm by the decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to AST activity in the sample.

Aspartate aminotransferase (AST/GOT) catalyzes the transfer of the amino group from aspartate to alpha-ketoglutarate, forming glutamate and oxaloacetate. The latter is reduced to malate by malate dehydrogenase (MDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH). The reaction is kinetically monitored at 340 nm by the decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to AST activity in the sample.

Aspartate aminotransferase (AST/GOT) catalyzes the transfer of the amino group from aspartate to alpha-ketoglutarate, forming glutamate and oxaloacetate. The latter is reduced to malate by malate dehydrogenase (MDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH). The reaction is kinetically monitored at 340 nm by the decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to AST activity in the sample.

Aspartate aminotransferase (AST/GOT) catalyzes the transfer of the amino group from aspartate to alpha-ketoglutarate, forming glutamate and oxaloacetate. The latter is reduced to malate by malate dehydrogenase (MDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH). The reaction is kinetically monitored at 340 nm by the decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to AST activity in the sample.

The method is based on the hydrolysis of a-naphthyl phosphate at pH 5.0 by acid phosphatase (FAC), producing a-naphthol and inorganic phosphate. Pentanediol acts as a phosphate acceptor, increasing the reaction sensitivity. a-naphthol reacts with a diazonium salt, Fast Red TR*, forming a colored complex directly proportional to the FAC activity in the sample.

The method is based on the specific binding of bromocresol green (VBC), an anionic dye, and the protein at an acidic pH, resulting in a shift in the absorption spectrum of the complex. The intensity of the formed color is proportional to the concentration of albumin in the sample.

The method is based on the specific binding of bromocresol green (VBC), an anionic dye, and the protein at an acidic pH, resulting in a shift in the absorption spectrum of the complex. The intensity of the formed color is proportional to the concentration of albumin in the sample.

The method is based on the specific binding of bromocresol green (VBC), an anionic dye, and the protein at an acidic pH, resulting in a shift in the absorption spectrum of the complex. The intensity of the formed color is proportional to the concentration of albumin in the sample.