Clonatest

Reagents for the calibration of quantitative determinations of clinical chemistry parameters. The use of a standard to calculate results allows for accuracy independent of the photometer and reading system used.

Reagents for the calibration of quantitative determinations of clinical chemistry parameters. The use of a standard to calculate results allows for accuracy independent of the photometer and reading system used.

LINEAR Human Multisera Normal is stabilized and lyophilized human origin serum. This control is prepared from blood of voluntary donors from different Blood Banks. Analytical data for Human Multisera Normal have been developed in collaboration between prestigious laboratories

Uric acid is oxidised by the action of uricase to allantoin and hydrogen peroxide. In the presence of peroxidase (POD) the mixture of dichlorophenol sulphonate (DCBS) and 4-aminoantipyrine (4-AA) are condensed by the action of hydrogen peroxide, forming a coloured quinonaimine proportional to the concentration of uric acid in the sample.

Urea is hydrolysed by urease to ammonia and carbon dioxide. Ammonia is converted to glutamate by glutamate dehydrogenase (GlDH) in the presence of NADH and ketoglutarate. The reaction is measured kinetically at 340 nm through the decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to the concentration of urea present in the sample.

Uric acid is oxidised by the action of uricase to allantoin and hydrogen peroxide. In the presence of peroxidase (POD) the mixture of dichlorophenol sulphonate (DCBS) and 4-aminoantipyrine (4-AA) are condensed by the action of hydrogen peroxide, forming a coloured quinonaimine proportional to the concentration of uric acid in the sample.

Uric acid is oxidised by the action of uricase to allantoin and hydrogen peroxide. In the presence of peroxidase (POD) the mixture of dichlorophenol sulphonate (DCBS) and 4-aminoantipyrine (4-AA) are condensed by the action of hydrogen peroxide, forming a coloured quinonaimine proportional to the concentration of uric acid in the sample.

Reagents for the calibration of quantitative determinations of clinical chemistry parameters. The use of a standard to calculate results allows for accuracy independent of the photometer and reading system used.

Reagents for the calibration of quantitative determinations of clinical chemistry parameters. The use of a standard to calculate results allows for accuracy independent of the photometer and reading system used.

En la reacción del biuret se forma un quelato entre el ión Cu2+ y los enlaces peptídicos de las proteínas en medio alcalino con la formación de un complejo coloreado violeta cuya absorbancia se mide fotométricamente. La intensidad del color producido es proporcional a la concentración de proteínas en la muestra.

The method is based on the enzymatic hydrolysis of serum triglycerides to glycerol and free fatty acids (FFA) by the action of lipoprotein lipase (LPL). Glycerol is phosphorylated by adenosine triphosphate (ATP) in the presence of glycerol kinase (GK) to form glycerol-3-phosphate (G-3-P) and adenosine diphosphate (ADP). G-3-P is oxidized by glycerophosphate oxidase (GPO) to dihydroxyacetone phosphate (DHAP) and hydrogen peroxide. In the presence of peroxidase (POD)

En la reacción del biuret se forma un quelato entre el ión Cu2+ y los enlaces peptídicos de las proteínas en medio alcalino con la formación de un complejo coloreado violeta cuya absorbancia se mide fotométricamente. La intensidad del color producido es proporcional a la concentración de proteínas en la muestra.