TPHA (Treponema Pallidum Hemagglutination) is a specific microplate hemagglutination test for the qualitative and semiquantitative detection of anti-Treponema pallidum antibodies in human serum. Stabilized and sensitized bird red blood cells with a T. pallidum antigenic solution agglutinate in the presence of anti-T.pallidum antibodies, showing characteristic agglutination patterns.