The method is based on the segmentation of the specific chromogenic substrate 1,2-O-dilaurylrac-glycero-3-glutaric- (6'methyl-resorufin)-ester emulsified in stabilising micro-particles. In the presence of specific pancreatic lipase activators such as colipase, calcium ions and bile acids, the substrate is transformed into 1,2-O-dilauryl-rac-glycerol and glutaric acid-6′-methylresorufinester which spontaneously decomposes to glutaric acid and methylresorufin. The intensity of the colour formed is proportional to the concentration of lipase in the sample.
