This technique employs a separation method based on the selective precipitation of apoprotein-B-containing lipoproteins (VLDL, LDL and (a)Lpa) by the action of phosphotungstic acid/Cl2Mg, sedimentation of the precipitate by centrifugation and subsequent enzymatic analysis as residual cholesterol from the high-density lipoproteins (HDL) contained in the clear supernatant.
