Gamma-glutamyltransferase (g-GT) catalyses the transfer of the g-glutamyl group from g-glutamyl-3-carboxy-4-nitroanilide to glycylglyclycine with the formation of L-g-glutamyl- glycylglycine and 5-amino-2-nitrobenzoate. The amount of 5-amino-2-nitrobenzoate formed, kinetically monitored at 405 nm, is proportional to the g-GT activity present in the sample.
