The respiPig 1 real time RT-PCR Kit is an assay for the simultaneous detection of the RNA of the North American (NA), European (EU) genotypes of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), the RNA of type A influenza viruses (Flu A) and the DNA of Mycoplasma hyopneumoniae (M. hyopneumoniae). Extracted RNA and DNA from swine blood, serum, tissue, bronchial swabs, saliva, bronchial lavage, semen, and cell culture supernatant samples can be used for analysis.